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micro sample genomic dna extraction kit  (tiangen biotech co)


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    tiangen biotech co micro sample genomic dna extraction kit
    Micro Sample Genomic Dna Extraction Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 11792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro+sample+genomic+dna+extraction+kit/Genomic+DNA+Kit/pmc12812391-100-12-18
    Average 96 stars, based on 11792 article reviews
    micro sample genomic dna extraction kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Integration of chromosomal microarray analysis and whole-exome sequencing for prenatal diagnosis of fetuses with cardiac ultrasound anomalies
    Article Snippet: The Affymetrix CytoScanTM 750K array (Applied Biosystems, USA) was used for chromosomal microarray analysis following the manufacturer’s instructions. .. In brief, genomic DNA from amniotic fluid cells was extracted using the Micro Sample Genomic DNA Extraction Kit (TIANGEN, China). ..

    Article Title: Predictive performance of Metagenomic Next Generation Sequencing in early detection of post-liver transplantation infections
    Article Snippet: .. Nucleic acid extraction was performed using the Micro-sample Genomic DNA Extraction Kit (DP316, Tiangen). .. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs Inc.), and sequencing was conducted on a Nextseq 550 DX platform (75 bp single-end reads).

    Article Title: Metagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis
    Article Snippet: .. The mNGS of CSF was meticulously carried out through the following streamlined process: (1) Specimen Collection: A volume of 1-2 mL of CSF was collected from patients via lumbar puncture, aliquoted into test tubes, and immediately stored at –80°C for 30 minutes prior to being utilized for mNGS. (2) Sample Extraction and Quality Control: Genomic DNA was extracted from the CSF samples using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China). .. The DNA was then fragmented into 200-300 base pair (bp) fragments using a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium).

    Article Title: Clinical application of metagenomic next-generation sequencing in rapid diagnosis and prognostic assessment of herpes simplex encephalitis
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1–2 mL of CSF was gathered, aliquoted, and preserved at −80°C for 30 min prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200–300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). ..

    Article Title: Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR.
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1-2 mL of CSF was gathered, aliquoted, and preserved at –80 ◦C for 30 minutes prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200-300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). .. Fragment sizes were evaluated with a 2100 Bioanalyzer, and DNA library concentration was quantified by qPCR. (3) Library Construction: DNA libraries were assembled with end-repair, poly(A)-tailing, adapter ligation, and PCR amplification, followed by roller amplification by 2-3 fold to create DNA nanospheres. (4) Sequencing: DNA nanospheres were sequenced on the BGISEQ-50 platform at Beijing Golden Key Gene Technology Co., Ltd. Bioinformatics Analysis: The raw sequencing data were converted into the fastq (fq) format for downstream data processing using the bcl2fastq software (Illumina, San Diego, CA).

    Article Title: MTFR1 phosphorylation-activated adaptive mitochondrial fusion is essential for colon cancer cell survival during glucose deprivation
    Article Snippet: One aliquot was retained for intact cells, while the other was processed using the Mitochondria Isolation Kit (Beyotime, C3601) according to the manufacturer's protocol to obtain the cytosolic fraction. .. DNA was extracted from both fractions using the Tiangen Micro Sample Genomic DNA Extraction Kit (Tiangen, DP316). ..

    Article Title: Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of VZV-Associated Rhombencephalitis.
    Article Snippet: .. 2.2.1 NGS of Cerebrospinal Fluid NGS of Cerebrospinal fluid: (1) Specimen collection: 1–2 mL cerebrospinal fluid was obtained by lumbar puncture in patients, dripped into test tubes and stored in a refrigerator at –80 °C for 30 min and used for NGS assays. (2) Sample extraction and quality control: The DNA of cerebrospinal fluid samples was extracted using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China), and fragmented into 200–300 bp fragments by DNA cutting ultrasonic disruptor(Bioruptor Pico, Diagenode, Belgium). ..

    Article Title: MTFR1 phosphorylation-activated adaptive mitochondrial fusion is essential for colon cancer cell survival during glucose deprivation.
    Article Snippet: Background: Mitochondrial dynamics are essential for maintaining cellular function under metabolic stress.. However, their role in colon cancer’s response to glucose deprivation remains poorly understood.. Methods: The role of the mitochondrial protein MTFR1 in colon cancer proliferation was evaluated using CCK-8 and colony formation assays.

    Extraction:

    Article Title: Predictive performance of Metagenomic Next Generation Sequencing in early detection of post-liver transplantation infections
    Article Snippet: .. Nucleic acid extraction was performed using the Micro-sample Genomic DNA Extraction Kit (DP316, Tiangen). .. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs Inc.), and sequencing was conducted on a Nextseq 550 DX platform (75 bp single-end reads).

    Article Title: Metagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis
    Article Snippet: .. The mNGS of CSF was meticulously carried out through the following streamlined process: (1) Specimen Collection: A volume of 1-2 mL of CSF was collected from patients via lumbar puncture, aliquoted into test tubes, and immediately stored at –80°C for 30 minutes prior to being utilized for mNGS. (2) Sample Extraction and Quality Control: Genomic DNA was extracted from the CSF samples using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China). .. The DNA was then fragmented into 200-300 base pair (bp) fragments using a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium).

    Article Title: Clinical application of metagenomic next-generation sequencing in rapid diagnosis and prognostic assessment of herpes simplex encephalitis
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1–2 mL of CSF was gathered, aliquoted, and preserved at −80°C for 30 min prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200–300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). ..

    Article Title: Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR.
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1-2 mL of CSF was gathered, aliquoted, and preserved at –80 ◦C for 30 minutes prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200-300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). .. Fragment sizes were evaluated with a 2100 Bioanalyzer, and DNA library concentration was quantified by qPCR. (3) Library Construction: DNA libraries were assembled with end-repair, poly(A)-tailing, adapter ligation, and PCR amplification, followed by roller amplification by 2-3 fold to create DNA nanospheres. (4) Sequencing: DNA nanospheres were sequenced on the BGISEQ-50 platform at Beijing Golden Key Gene Technology Co., Ltd. Bioinformatics Analysis: The raw sequencing data were converted into the fastq (fq) format for downstream data processing using the bcl2fastq software (Illumina, San Diego, CA).

    Article Title: Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of VZV-Associated Rhombencephalitis.
    Article Snippet: .. 2.2.1 NGS of Cerebrospinal Fluid NGS of Cerebrospinal fluid: (1) Specimen collection: 1–2 mL cerebrospinal fluid was obtained by lumbar puncture in patients, dripped into test tubes and stored in a refrigerator at –80 °C for 30 min and used for NGS assays. (2) Sample extraction and quality control: The DNA of cerebrospinal fluid samples was extracted using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China), and fragmented into 200–300 bp fragments by DNA cutting ultrasonic disruptor(Bioruptor Pico, Diagenode, Belgium). ..

    Clinical Proteomics:

    Article Title: Metagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis
    Article Snippet: .. The mNGS of CSF was meticulously carried out through the following streamlined process: (1) Specimen Collection: A volume of 1-2 mL of CSF was collected from patients via lumbar puncture, aliquoted into test tubes, and immediately stored at –80°C for 30 minutes prior to being utilized for mNGS. (2) Sample Extraction and Quality Control: Genomic DNA was extracted from the CSF samples using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China). .. The DNA was then fragmented into 200-300 base pair (bp) fragments using a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium).

    Article Title: Clinical application of metagenomic next-generation sequencing in rapid diagnosis and prognostic assessment of herpes simplex encephalitis
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1–2 mL of CSF was gathered, aliquoted, and preserved at −80°C for 30 min prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200–300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). ..

    Article Title: Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR.
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1-2 mL of CSF was gathered, aliquoted, and preserved at –80 ◦C for 30 minutes prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200-300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). .. Fragment sizes were evaluated with a 2100 Bioanalyzer, and DNA library concentration was quantified by qPCR. (3) Library Construction: DNA libraries were assembled with end-repair, poly(A)-tailing, adapter ligation, and PCR amplification, followed by roller amplification by 2-3 fold to create DNA nanospheres. (4) Sequencing: DNA nanospheres were sequenced on the BGISEQ-50 platform at Beijing Golden Key Gene Technology Co., Ltd. Bioinformatics Analysis: The raw sequencing data were converted into the fastq (fq) format for downstream data processing using the bcl2fastq software (Illumina, San Diego, CA).

    Control:

    Article Title: Metagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis
    Article Snippet: .. The mNGS of CSF was meticulously carried out through the following streamlined process: (1) Specimen Collection: A volume of 1-2 mL of CSF was collected from patients via lumbar puncture, aliquoted into test tubes, and immediately stored at –80°C for 30 minutes prior to being utilized for mNGS. (2) Sample Extraction and Quality Control: Genomic DNA was extracted from the CSF samples using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China). .. The DNA was then fragmented into 200-300 base pair (bp) fragments using a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium).

    Article Title: Clinical application of metagenomic next-generation sequencing in rapid diagnosis and prognostic assessment of herpes simplex encephalitis
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1–2 mL of CSF was gathered, aliquoted, and preserved at −80°C for 30 min prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200–300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). ..

    Article Title: Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR.
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1-2 mL of CSF was gathered, aliquoted, and preserved at –80 ◦C for 30 minutes prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200-300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). .. Fragment sizes were evaluated with a 2100 Bioanalyzer, and DNA library concentration was quantified by qPCR. (3) Library Construction: DNA libraries were assembled with end-repair, poly(A)-tailing, adapter ligation, and PCR amplification, followed by roller amplification by 2-3 fold to create DNA nanospheres. (4) Sequencing: DNA nanospheres were sequenced on the BGISEQ-50 platform at Beijing Golden Key Gene Technology Co., Ltd. Bioinformatics Analysis: The raw sequencing data were converted into the fastq (fq) format for downstream data processing using the bcl2fastq software (Illumina, San Diego, CA).

    Article Title: Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of VZV-Associated Rhombencephalitis.
    Article Snippet: .. 2.2.1 NGS of Cerebrospinal Fluid NGS of Cerebrospinal fluid: (1) Specimen collection: 1–2 mL cerebrospinal fluid was obtained by lumbar puncture in patients, dripped into test tubes and stored in a refrigerator at –80 °C for 30 min and used for NGS assays. (2) Sample extraction and quality control: The DNA of cerebrospinal fluid samples was extracted using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China), and fragmented into 200–300 bp fragments by DNA cutting ultrasonic disruptor(Bioruptor Pico, Diagenode, Belgium). ..

    Isolation:

    Article Title: Clinical application of metagenomic next-generation sequencing in rapid diagnosis and prognostic assessment of herpes simplex encephalitis
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1–2 mL of CSF was gathered, aliquoted, and preserved at −80°C for 30 min prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200–300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). ..

    Article Title: Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR.
    Article Snippet: .. The CSF mNGS procedure unfolded as follows: (1) Specimen Collection: 1-2 mL of CSF was gathered, aliquoted, and preserved at –80 ◦C for 30 minutes prior to mNGS analysis. (2) Sample Extraction and Quality Control: Genomic DNA was isolated using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China) and fragmented to 200-300 base pairs (bp) utilizing a DNA cutting ultrasonic disruptor (Bioruptor Pico, Diagenode, Belgium). .. Fragment sizes were evaluated with a 2100 Bioanalyzer, and DNA library concentration was quantified by qPCR. (3) Library Construction: DNA libraries were assembled with end-repair, poly(A)-tailing, adapter ligation, and PCR amplification, followed by roller amplification by 2-3 fold to create DNA nanospheres. (4) Sequencing: DNA nanospheres were sequenced on the BGISEQ-50 platform at Beijing Golden Key Gene Technology Co., Ltd. Bioinformatics Analysis: The raw sequencing data were converted into the fastq (fq) format for downstream data processing using the bcl2fastq software (Illumina, San Diego, CA).

    Next-Generation Sequencing:

    Article Title: Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of VZV-Associated Rhombencephalitis.
    Article Snippet: .. 2.2.1 NGS of Cerebrospinal Fluid NGS of Cerebrospinal fluid: (1) Specimen collection: 1–2 mL cerebrospinal fluid was obtained by lumbar puncture in patients, dripped into test tubes and stored in a refrigerator at –80 °C for 30 min and used for NGS assays. (2) Sample extraction and quality control: The DNA of cerebrospinal fluid samples was extracted using a micro-sample genomic DNA extraction kit (DP316, TIANGEN BIOTECH, Beijing, China), and fragmented into 200–300 bp fragments by DNA cutting ultrasonic disruptor(Bioruptor Pico, Diagenode, Belgium). ..



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